Diauxie used as a microbiological assay for L-isoleucine or L-valine in Escherichia coli
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Diauxie used as a microbiological assay for L-isoleucine or L-valine in Escherichia coli.
FIG. 1. Diauxic lag of cuiltures conitaining valine and isoleucine. All cultures were grown by shakinig (330 rev/ min) at 37 C in medium containing 2.0 g ofNaH2PO4 O H20, 7.0 g ofK2HP04, 2.0 g of (NH4)2SO4, 0.2 g of MgSO4 v 7H20 and I mg of thiamine hydrochloride in I liter of distilled water. Overnight cultures grown in 0.2%o glucose were harvested by centrifugation during exponential phase, r...
متن کاملIsoleucine and Valine Metabolism of Escherichia Coli
In the preceding publication (1) mention has been made of the syntrophism existing between the mutant strains llA16 and 2OA19 of Escherichia COG. These mutants, requiring isoleucine and isoleucine plus valine respectively, were able to grow together on a minimal agar plate, free of amino acids, which would not support the growth of either mutant alone. The nature of this syntrophism forms the s...
متن کاملIsoleucine and valine metabolism in Escherichia coli. V. Antagonism between isoleucine and valine.
The influence of the relative concentrations of amino acids in culture media on the growth of microorganisms was first brought to the attention of microbiologists by Gladstone (1939). He observed that isoleucine, valine, or leucine added singly to a synthetic medium inhibited the growth of the anthrax bacillus whereas a mixture of the three stimulated growth. Although Gladstone was unable at th...
متن کاملIsoleucine and Valine Metabolism in Escherichia coZi
On the basis of isotopic results obtained with Torulopsis utilis and Neurospora crassa, Strassman et al. (1) and Adelberg (2) have proposed a mechanism for valine biosynthesis in which pyruvate is converted to a-acetolactate. Acetolactate would then undergo a rearrangement to yield the carbon skeleton of valine. Abelson (3) showed that pyruvate, in competition with glucose, gave rise to the car...
متن کاملGlucose-lactose diauxie in Escherichia coli.
Growth of Escherichia coli in medium containing glucose, at a concentration insufficient to support full growth, and containing lactose, is diauxic. A mutation in the gene, CR, which determines catabolite repression specific to the lac operon, was found to relieve glucose-lactose but not glucose-maltose diauxie. Furthermore, a high concentration of lactose was shown to overcome diauxie in a CR(...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1968
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.95.5.1964-1965.1968